Cat. No.
MABL-1379
Application
IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
AA12
From
Recombinant Antibody
Specificity
This antibody recognises all mammalian homologs of alpha tubulin, as well as detyrosinated alpha tubulin, a post-translational modification of alpha tubulin.
Alternative Names
detyrosinated α-tubulin
UniProt
Immunogen
This antibody was raised against alpha tubulin (GEEEGE) in BALB/c mice.
Application Notes
This antibody has been used, as part of a panel, in Western blot analysis of the distribution pattern of tubulin subtypes in whole-cell lysates of human ovarian cancer A2780-TC1 cells (Esteve et al, 2006) and of the proteins extracted from lentiviral-infected Duchenne muscular dystrophy (Δ48–50) myoblasts (Incitti et al, 2010).
Antibody First Published
Esteve et al. Bcl-2 down-regulation and tubulin subtype composition are involved in resistance of ovarian cancer cells to vinflunine. Mol Cancer Ther. 2006 Nov;5(11):2824-33. PMID: 17121929
Note on publication
Describes the original use of this antibody in Western blot analysis of tubulin subtype expression patterns.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
