Cat. No.
MABL-1381
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
12C2
From
Recombinant Antibody
Specificity
The antibody is specific for a broad set of O,O-diethyl organophosphorus pesticides (DOPs). DOPs are widely used for agricultural and landscape pest control. However, it has been reported that OPs are harmful to human health though food exposure.
Alternative Names
O,O-diethyl organophosphorus pesticides; DOPs; OPs
UniProt
Immunogen
The antibody was generated by immunizing BABL/c mice with the diethoxyphosphorothioate derivated hapten, followed by antibody library construction and phage display-based selection.
Application Notes
The specificity of this antibody for a broad set of O,O-diethyl organophosphorus pesticides (DOPs) was confirmed by indirect competitive ELISA. IC50 of the Fab antibody was 135.7ng/mL. A standard curve showed that the linear detection range is 7.2ng/mL-861.7ng/mL. The Fab fragment detected soluble DOPs by western blot (Chen et al., 2018;PMID: 29734787). The specificity of the original format of the antibody was confirmed by competitive indirect ELISA analysis (Xu et al. 2010; PMID: 20958019). The original format of the antibody was linked to alkaline phosphatase (scFv−AP) and employed to develop a competitive direct enzyme-linked immunosorbent assay (cdELISA) for O,O-diethyl OPs in environmental and agricultural samples (Xu et al. 2012; PMID: 22533788).
Antibody First Published
Chen et al. Production of Antigen-Binding Fragment against O,O-Diethyl Organophosphorus Pesticides and Molecular Dynamics Simulations of Antibody Recognition. Int J Mol Sci. 2018 May; 19(5): 1381. PMID: 29734787
Note on publication
The paper describes the generation and characterization of the Fab fragment. The antibody reacts against a broad set of O,O-diethyl organophosphorus pesticides (DOPs). The 3D model of Fab was built via homologous modeling and the interaction between Fab and DOPs was studied via molecular docking and dynamics simulations.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
