Cat. No.
MABL-1383
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
19-11
From
Recombinant Antibody
Specificity
This antibody is specific for digoxigenin A. Digoxigenin (DIG) is a hydroxy steroid that consists of 5beta-cardanolide, found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata. It is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications.
Alternative Names
Digoxigenin; DOG; DIG; ChEBI: 42098; CAS:1672-46-4
UniProt
Immunogen
The original antibody was generated by immunizing mice with digoxin conjugated to human serum albumin.
Application Notes
This antibody can be used for the detection of digoxigenin via ELISA. This antibody was also used for the generation of bispecific antibodies that bind cell-surface targets as well as digoxigenin (Dig) for targeted payload delivery (PMID: 21536919).
Antibody First Published
Metz et al. Bispecific digoxigenin-binding antibodies for targeted payload delivery. Proc Natl Acad Sci U S A. 2011 May 17; 108(20): 8194–8199. PMID: 21536919
Note on publication
Describes the generation of bispecific antibodies for targeted payload delivery.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
