Cat. No.
MABL-1384
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
Clone 3
From
Recombinant Antibody
Specificity
This antibody is specific for digoxigenin A. Digoxigenin (DIG) is a hydroxy steroid that consists of 5beta-cardanolide, found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata. It is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications.
Alternative Names
Digoxigenin; DigA; DOG; ChEBI: 42098; CAS: 1672-46-4
UniProt
Immunogen
The original antibody was isolated from a IgM-derived scFv phage antibody library created from the peripheral blood lymphocytes of 20 non-immunized doners. The specific clone was isolated by panning the library against digoxigenin.
Application Notes
The binding specificity of this antibody for digoxigenin was checked using ELISA. This antibody did not bind digoxigenin coupled by a methylcarbonyl (Dig-Met) or succinyl (Dig-Suc) type linker to BSA (PMID: 9305722).
Antibody First Published
Dörsam et al. Antibodies to steroids from a small human naive IgM library. FEBS Lett. 1997 Sep 1;414(1):7-13. PMID: 9305722
Note on publication
Describes the generation of antibodies against steroids from a human naive IgM library.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
