Cat. No.
MABL-1394
Application
Immunoperoxidase staining, RIA, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
ME36.1
From
Recombinant Antibody
Specificity
This antibody binds both GD2 and GD3. ME36.1 has higher affinity for GD2 than GD3.
Alternative Names
GD2; GD3
UniProt
Immunogen
The original antibody was generated by immunizing BLAB/c mice with cells of metastatic melanoma SK MEL 23 cell culture and boosted again four weeks later.
Application Notes
The disialoganglioside GD2 is expressed on the surface of cells of most human melanomas but not on the surface of melanocytes. The specificty of this antibody towards ganglioside antigens GD2 and GD3 was demonstrated using solid-phase radioimmunoassay and enzyme-linked immunosorbent assay as well as by immunostaining on thin-layer chromatography plates using structurally characterized gangliosides. Binding of antibody to live cells was done by flow cytometry and by immunoperoxidase staining on frozen tissue sections. The binding patterns correlated with antitumor activity in antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity assays with human effector cells and complement in an release assay using cell lines derived from the same individual. (PMID: 3815333). This antibody was also used in monitering the levels of GD2 and GD3 in the sera of patients suffering from melanoma and healthy adults (PMID: 2778837)
Antibody First Published
Thurin et al. Monoclonal antibody-defined correlations in melanoma between levels of GD2 and GD3 antigens and antibody-mediated cytotoxicity. Cancer Res. (1987);4 7(5):1229-33. PMID: 3815333
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
