Cat. No.
MABL-1396
Application
RIA, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3G6
From
Recombinant Antibody
Specificity
This antibody binds the GD2 antigen, but does not cross react with GD3, GT1b, GD1b, GD1a, GM1, GM3 and GM4.
Alternative Names
GD2
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with human neuroblastoma cells.
Application Notes
The binding studies of this antibody to various human tissues was done using ELISA and indirect immunofluorescence. This antibody also showed comparable reciprocal cross-blocking in radioimmunoassay on LAN-1 NB cells or NB glycolipid extracts (PMID: 2580625). This antibody is also recommended for the study of distribution of the GD2 antigen on various types of cancer tissues. This antibodhy was also used in the development of a chimeric receptor 3G6-CD28, which provides CD28 signaling upon specific recognition of the GD2 antigen on tumor cells (PMID: 9705944). Expression of GD2 expressing cells was done by flow cytometry using 3G6. This antibody was also used in anti-GD2 therapy to test the survival and metastasis after challenge with EL4 cells (PMID: 16288049).
Antibody First Published
Cheung et al. Monoclonal antibodies to a glycolipid antigen on human neuroblastoma cells. Cancer Res. (1985) Jun;45(6):2642-9. PMID:2580625
Note on publication
Describes the generation of five antibodies against a human neuroblastoma cell surface glycolipid antigen.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
