Cat. No.
MABL-1397
Application
therapeutic, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
5F11
From
Recombinant Antibody
Specificity
This antibody binds the GD2 antigen, but does not cross react with GD3, GT1b, GD1b, GD1a, GM1, GM3 and GM4.
Alternative Names
GD2
UniProt
Immunogen
The original antibody was generated by immunizing mice with ganglioside G.sub.D2..sup.9.
Application Notes
This antibody is recommended to be used in the detection of GD2 positive tumor cells in the marrow specimens using immunofluorescence staining (PMID: 15136638). It is also recommended for staining of neuroblastoma cells and analysing them using flow cytometry (PMID: 2526848). The anti-GD2 scFv 5F11 recombinantly fused to streptavidin (5F11-scFv-SA) formed a homo-tetramer and showed high GD2 binding. 5F11-scFv-SA allowed inhibition of the binding of anti-5F11 antiidiotypic antibody 1G8 to 5F11 in an enzyme-linked immunosorbent assay (PMID: 15136638). Diphtheria toxins fused to scFv 5F11 demonstrated specific killing of GD2+ tumor cells independent of host immune function (PMID: 11877684). This antibody was also used for the development of bispecific antibody (5F11-scBA) to target disialoganglioside (GD2) positive tumors (PMID: 27471647).
Antibody First Published
Cheung et al. Single-chain Fv-streptavidin substantially improved therapeutic index in multistep targeting directed at disialoganglioside GD2. J Nucl Med. (2004); 45(5):867-77. PMID: 15136638
Note on publication
Describes the use of a homotetramer 5F11-scFv-streptavidin in multistep targeting towards disialoganglioside GD2 to improve the therapeutic index of antibody based targeting.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
