Cat. No.
MABL-1400
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HP-92
From
Recombinant Antibody
Specificity
This antibody binds human Delta-like protein 3 (DLL3), which is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL3 consists of a 466 amino acid (aa) extracellular domain (ECD) with one DSL domain and six EGF-like repeats, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain. It is an inhibitory Notch ligand that is highly expressed in small cell lung cancer (SCLC) and other neuroendocrine tumors but minimally expressed in normal tissues. It is therefore being explored as a potential therapeutic target in SCLC.
Alternative Names
Delta-like protein 3; Drosophila Delta homolog 3 (Delta3)
UniProt
Q9NYJ7
Immunogen
The original antibody was generated by immunizing two alpacas with human DLL3.
Application Notes
The binding affinity to human DLL3 that is comparable with Rovalpituzumab. The binding characterization of this antibody for human DLL3 was done using ELISA. This antibody was capable of binding DLL3 expressed on the surface of H446 and H524 small cell lung cancer cell lines using flow cytometry (CN114057880).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
