Cat. No.
MABL-1399
Application
Blocking, Functional Studies, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
HMD1-5
From
Recombinant Antibody
Specificity
The antibody HMD1-5 recognizes Dll1 which is a one of five type I transmembrane proteins that serves as a Notch receptor ligand.
Alternative Names
Delta1; Delta-like protein 1; Drosophila Delta homolog 1
UniProt
Q61483
Immunogen
The original hamster IgG version of this antibody was raised by immunizing Armenian hamsters (Oriental Yeast) with Dll1-expressing CHO cells via intraperitoneal injections and subsequent generation of the hybridoma cells.
Application Notes
This antibody can be used to investigate the role of the signalling pathways related to the Notch receptors and its ligands. The original hamster IgG version of this antibody was successfully used as a blocking agent to inhibit the binding of Notch2-Fc to Dll1/CHO cells (Moriyama et al., 2008; pmid: 18381350), demonstrating that this clone is an effective inhibitor of the interactions between Dll1 and Notch receptors. Another study investigated the role of Delta1 in allograft rejection by blocking it with HMD1-5 (Riella et al., 2011; pmid: 21949024). This blocking ability was further corroborated by showing that HMD1-5 stops the Dll1-Fc-triggered Notch2 signaling (Moriyama et al., 2008; pmid: 18381350). Furthermore, this clone was utilized in flow cytometry to analyse the Notch ligands' expression on splenocytes, monocytes and macrophages (Moriyama et al., 2008; pmid: 18381350; Sekine et al., 2009; 19181931). Similarly, HMD1-5 has also detected Dll1-expressing cells in spleen sections in immunohistochemistry (Moriyama et al., 2008; pmid: 18381350; Sekine et al., 2009; 19181931). Finally, this antibody can be used to assess the role of Dll1 signalling and the role of the Notch receptors in various settings. The same group used this clone to investigate the involvement of Dll1 in the maintenance of MZBcells (Marginal zone B cells) (Moriyama et al., 2008; pmid: 18381350).
Antibody First Published
Moriyama et al. Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice Int Immunol. 2008 Jun;20(6):763-73. doi: 10.1093/intimm/dxn034. Epub 2008 Apr 1. PMID: 18381350
Note on publication
Describes the generation and characterization of the HMD1-5 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
