Cat. No.
MABL-1404
Application
functional assay, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
SC16.25
From
Recombinant Antibody
Specificity
This antibody binds human DLL3 in the N-terminal domain. Cross-reacts with cynomolgus monkey DLL3 with no binding to mouse/rat DLL3. Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL3 consists of a 466 amino acid (aa) extracellular domain (ECD) with one DSL domain and six EGF-like repeats, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain. It is an inhibitory Notch ligand that is highly expressed in small cell lung cancer (SCLC) and other neuroendocrine tumors but minimally expressed in normal tissues. It is therefore being explored as a potential therapeutic target in SCLC.
Alternative Names
Delta-like protein 3; Drosophila Delta homolog 3 (Delta3)
UniProt
Q9NYJ7
Immunogen
The original antibody was generated by immunizing three strains of mice (Balb/c, CD-1 and FVB) with recombinant human DLL3-Fc or with human DLL3-His fusion constructs.
Application Notes
The binding characterization of this antibody to human DLL3 was done using ELISA. Binding affinity of mouse IgG antibody for human DLL3 is Kd= less than 0.2 nM. This antibody can bind human and cynomolgus monkey DLL3 expressed on surface of 293 cells via flow cytometry. This antibody can mediate killing of tumor cells expressing DLL3. Humanized version of this antibody is also available on request (US9089615).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
