Cat. No.
MABL-1413
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
5C2
From
Recombinant Antibody
Specificity
This antibody binds human topoisomerase 1. This enzyme controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA.
Alternative Names
TOP1; Topo; DNA topoisomerase I
UniProt
P11387
Immunogen
The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
Application Notes
The binding characterization of this antibody was done using ELISA (PMID: 18062792).
Antibody First Published
Hof et al. Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens. Clin Exp Immunol. 2008 Feb; 151(2): 367–377. PMID: 18062792
Note on publication
Describes the generation of chicked antibodies against various human autoantigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
