Cat. No.
MABL-1412
Application
ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
4B1
From
Recombinant Antibody
Specificity
This antibody is a polyspecific antibody that has binding specificity for ssDNA, actin, tubulin, cardiolipin and laminin.
Alternative Names
ssDNA; actin; tubulin; cardiolipin; laminin.
UniProt
Immunogen
The original antibody was derived from a 12 month old (NZB × NZW)F1 lupus mouse
Application Notes
This antibody is capable of binding ssDNA, actin, tubulin, cardiolipin and to laminin in a solid phase ELISA. Indirect immunofluorescence labeling of HEp-2 cells gave a cytoplasmic staining pattern similar to that obtained with anti-cytoskeleton antibodies. Western blot analysis demonstrated that mAb 4B1 bore idiotype D23, previously shown to be characteristic of natural antibodies derived from normal mice. This antibody was able to form glomerular immune deposits (Gilbert et al., 1995; PMID: 7540257).
Antibody First Published
Gilbert et al. An idiotype D23-bearing polyspecific, murine anti-DNA monoclonal antibody forms glomerular immune deposits. Pathogenic role of natural autoantibodies? Mol Immunol. 1995 May;32(7):477-86. PMID:7540257
Note on publication
Describes the generation and characterization of a polyspecific anti-DNA antibody that forms glomerular immune deposits.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
