Cat. No.
MABL-1411
Application
IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Cow, Rat, Human, Mouse
Clone No.
2H12/4
From
Recombinant Antibody
Specificity
This antibody is specific for DMC1 and does not cross-react with the related protein Rad51. DMC1 is a RecA/RAD51 homologue which is specific for meiosis and critical for meiotic recombination in Eukaryota.
Alternative Names
Meiotic recombination protein DMC1/LIM15 homolog; DMC1H; LIM15; MGC150473; MGC150472; HsLim15; DMC1 homologue; disrupted meiotic cDNA1; hDMC1
UniProt
Q14565
Immunogen
This antibody was raised against human DMC1 protein (expressed originally as a 6xHis fusion in E. coli).
Application Notes
IF: 2H12/4 anti-DMC1 antibody was used to visualise DMC1 on chromatin in I-L2-NS endopolyploid tumour cells in experiments investigating the role of another protein - REC8 in chromosome segregation after radiation (Erenpreisa et al., 2009). This antibody was also utilised to stain paraffin- embedded cervical cancer tissue samples against DMC1 (Ianzini et al., 2009). IF: Hopkins et al. (2014) successfully used this antibody to stain chromatin spreads from purified testicular germ cells of Stag3+/− and Stag3−/− mice for DMC1 in the studies on the role of Stag3. IF: Another group also used this antibody for the immunofluorescence staining of spermatocyte chromosome spreads against DMC1 in a study analysing proteins involved in telomeres' attachment to the nuclear envelope (Wang et al., 2019).
Antibody First Published
Erenpreisa et al. The role of meiotic cohesin REC8 in chromosome segregation in γ irradiation-induced endopolyploid tumour cells Exp Cell Res. 2009 Sep 10;315(15):2593- 603. doi: 10.1016/j.yexcr.2009.05.011. Epub 2009 May 20. PMID: 19463812
Note on publication
This article describes the earliest use of this anti-DMC1 antibody. It was utilised to visualise DMC1 protein on chromosomes of endopolyploid tumour cells in experiments investigating the role of another protein - REC8 in chromosome segregation after radiation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
