Cat. No.
MABL-1440
Application
NTRL
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
YSd1
From
Recombinant Antibody
Specificity
YSd1 was selected during phage display on the basis of its binding to the extracellular domain of DR5. It binds to the N-terminal portion of the molecule, binding to the smaller of two binding sites for the natural DR5 ligand Apo2L/TRAIL in the same fashion as the natural ligand.
Alternative Names
TRAILR2; Death receptor 5; DR-5; TRAIL receptor 2; Tumour necrosis factor receptor superfamily member 10B; TNFRSF10B
UniProt
O14763
Immunogen
Fab fragments were generated by constructing phage-display libraries in which the CDRs of a humanised Fab framework derived from the murine Ab 4D5 were randomised (see Eigenbrot et al, 1993).The 1st and 2nd CDRs of the VH domain were replaced by randomised sequences consisting only of Tyr and Ser residues, whilst the 3rd VH CDR was substituted with random loops of variable lengths comprising all naturally occurring amino acids. Specific Fabs were isolated using ELISA screening.
Application Notes
YSd1 bound DR5 with high affinity, shown using competitive phage ELISA as well as surface plasmon resonance (with a Kd of 34 nM). By binding to interfacial regions of DR5 which are sites of physiological ligand interaction, it is likely that YSd1 has the capacity to neutralise DR5 activity by inhibition of downstream signalling.
Antibody First Published
Fellouse et al. Molecular recognition by a binary code. J Mol Biol. 2005 May 20;348(5):1153-62 PMID:15854651
Note on publication
Describes the generation of antibodies specific for human VEGF and human DR5, using the VH and VL framework regions of the murine anti-human HER2 Ab 4D5. The CDRs of the parental Ab were randomised using only Ser and Tyr residues, highlighting the potential to generate binding specificity from a limited chemical repertoire. The anti-DR5 Ab, YSd1, was crystallised with the N-terminal DR5 extracellular domain.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
