Cat. No.
MABL-1441
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
P2B1
From
Recombinant Antibody
Specificity
This antibody recognises C-terminal fragment of human DUX4-fl, aa 349-424. It does not cross- react with DUX4c. DUX4 is a protein thought to be engaged in facioscapulohumeral dystrophy (FSHD), one of the most common muscular dystrophies.
Alternative Names
Double homeobox protein 4; Double homeobox protein 10; DUX10; DUX4L1; P2B1-B3
UniProt
Q9UBX2
Immunogen
This antibody was raised by immunising mice with C-terminal fragment of human of DUX4 (aa 349-424) with glutathione-s-transferase (gst) tag.
Application Notes
P2B1 antibody can be used to assess the protein expression of DUX4 in muscle cells affected by facioscapulohumeral dystrophy (FSHD). For instance, this antibody was used to stain C2C12 myoblasts transfected with human DUX4 (Geng et al., 2011).
Antibody First Published
Geng et al. Immunodetection of human double homeobox 4. Hybridoma (Larchmt). 2011 Apr;30(2):125-30. doi: 10.1089/hyb.2010.0094. PMID:21529284
Note on publication
This article describes the generation and characterisation of the P2B1 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
