Cat. No.
MABL-1446
Application
IP, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Rat, Human
Clone No.
10F6-D1-D9
From
Recombinant Antibody
Specificity
This antibody is specific for DYNLT1 and DYNLT3 in both humans and rats. DYNLT1 and DYNLT3 act as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function.
Alternative Names
Dynein light chain Tctex-type 1; DYNLT1; Protein CW-1; T-complex testis- specific protein 1 homolog; Dynein light chain Tctex-type 3; DYNLT3; Protein 91/23; T-complex-associated testis-expressed 1-like
UniProt
P63172; P51808
Immunogen
The mouse version of this antibody was raised by immunizing mice with DYNLT1- and DYNLT3-GST fusion proteins.
Application Notes
To show the specificity of the mouse version of this antibody, immunoprecipitation and western blots on DYNLT1- and DYNLT3-GST fusion proteins were preformed with this antibody. Furthermore, the blotting and immunoprecipitating experiments were preformed on brain and tissue culture cell lysates and microtubule pellets prepared from rat brain. In both experiments it showed positive results. The antibody was isolated using ELISA on the DYNLT1- and DYNLT3-GST fusion proteins (Lo et al, 2007; pmid:17965411).
Antibody First Published
Lo et al. Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex J Biol Chem. 2007 Dec 21;282(51):36871-8. PMID:17965411
Note on publication
Researching the cytoplasmic dynein 1 cargo binding domain and determining how the subunit isoforms are assembled or if there is any specificity to their interactions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
