Cat. No.
MABL-1450
Application
agonist, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Dog, Human
Clone No.
19A11
From
Recombinant Antibody
Specificity
This antibody recognizes the N-terminal extracellular cadherin repeat domain 1 (EC1) of the human E-cadherin. This antibody also cross reacts with canine E-cadherin. Cadherins are calcium-dependent cell adhesion proteins. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. E-cadherins are reported to play an important role in cancer metastasis.
Alternative Names
CD324; Cadherin-1; Cadherin E; E-Cadherin; epithelial cadherin; CAM 120/80; Uvomorulin
UniProt
P12830
Immunogen
The original antibody was generated by immunizing mice with purified ECad1-5 protein.
Application Notes
The initial screeding of E-Cadherin–positive hybridoma supernatants for binding to live Colo 205 cells was done using an ELISA. Activating antibody 19A11 stimulated the adhesion of Colo 205 cells to pure E-cadherin substrate (PMID: 22513089). This antibody is also capable of recognizing denature human E-cadherin in a western blot. This antibody also inhibited the formation of epithelial tubules, causing the cells to remain as round epithelial cysts. This antibody also enhanced the adhesion of HGF-treated MDCK cells to E-cadherin when analyzed with the flow adhesion assay (PMID: 22513089). This antibody slowed the rate of wound closure in both normal culture medium and HGF-treated cultures (PMID: 22513089). This antibody is an E-cadherin activating antibody which stimulates the apoptosis of circulating tumor cells (PMID: 32127478). This antibody was used in determination of expression of E-cadherin on low- adherent Colo205 cells using flow cytometry (PMID: 27582386).
Antibody First Published
Petrova et al. Conformational epitopes at cadherin calcium-binding sites and p120-catenin phosphorylation regulate cell adhesion. Mol Biol Cell. 2012 Jun 1; 23(11): 2092–2108.PMID: 22513089
Note on publication
The paper investigated the change in cadherin structure at the cell surface that regulate its adhesive activity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
