Cat. No.
MABL-1458
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
E. coli
Clone No.
9A5
From
Recombinant Antibody
Specificity
The antibody recognizes LacI and recombinant proteins containing the LacI tag. The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. These genes are repressed when lactose is not available to the cell, ensuring that the bacterium only invests energy in the production of machinery necessary for uptake and utilization of lactose when lactose is present. When lactose becomes available, it is converted into allolactose, which inhibits the lac repressor's DNA binding ability. Loss of DNA binding by the lac repressor is required for transcriptional activation of the operon.
Alternative Names
Lactose operon repressor
UniProt
P03023
Immunogen
The original antibody was generated by immunizing mice against E.coli LacI.
Application Notes
This antibody was used for immunohistochemistry on tissue sections from mice transgenic for the R transgene (muscle, skin, hippocampus, cerebellum) (Cronin et al, 2001; PMID: 11410531). The antibody was used to detect the lac repressor protein by western blot analysis (Blevins et al., 2007; PMID: 17220265). The antibody was employed to stain third instar larval salivary glands (Hines et al., 2009; PMID: 19487560). Immunofluorescence staining using the antibody was performed on parental B49.5 lacO integrated cells and B49.5 cells stably expressing lacI-lap2b (Finlan et al., 2008; PMID: 18369458).
Antibody First Published
Cronin et al. The lac operator-repressor system is functional in the mouse. Genes Dev. 2001 Jun 15;15(12):1506-17. PMID:11410531
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
