Cat. No.
MABL-1457
Application
functional assay, therapeutical, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CY1787
From
Recombinant Antibody
Specificity
This antibody is specific for human E-selectin. E-selectin is a cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Alternative Names
SELE; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E
UniProt
Immunogen
The original mouse version of this antibody was raised by immunizing a mouse with E- selectin.
Application Notes
To test whether the recombinantly created e-selectin was expressed ELISA was preformed with the murine version of this antibody (WO1996040942A1). To test the binding specificity of the human version of this antibody an ELISA was preformed on soluble e-selectin (WO1996040942A1). Neutrophil adhesion to activated ECs under rotating conditions was assayed in the presence of the murine version of this antibody. Neutrophil adhesion to stimulated ECs under rotating conditions was completely inhibited by this antibody (Matsumiya et al, 1999; pmid:10069494). To determine the saferty and pharmokinetics of the murine version of this antibody in patients with newly developed septic shock. This antibody was given to nine patients that survived the first 24 hours of septic shock. This antibody was well tolerated in all patients. Signs of shock resolved in all patients, and organ failure entirely reversed in eight patients (Friedmann et al, 1996; pmid:8605793). To study e-selectin expression in a feline model of myocardial ischemia and reperfusion. The murine version of this antibody was used for immunohistochemistry on the heart and coronary arteries of male cats. (Weyrich et al, 1995; pmid:7530283).
Antibody First Published
Weyrich et al. Time course of coronary vascular endothelial adhesion molecule expression during reperfusion of the ischemic feline myocardium J Leukoc Biol. 1995 Jan;57(1):45-55. PMID: 7530283
Note on publication
The time course of endothelial P-selectin, ICAM-1, and E-selectin expression was studied in a feline model of myocardial ischemia and reperfusion.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
