Cat. No.
MABL-1461
Application
SPR
Isotype
Engineer antibody
Species Reactivity
WEEV
Clone No.
WD11F
From
Recombinant Antibody
Specificity
The antibody is specific for E2/E3E2 envelope protein.Two envelope proteins, glycoproteins E1 and E2, associate as trimers of E1-E2 dimers on the viral surface, and make up 80 spikes on the viral surface.
Alternative Names
Western equine encephalitis virus
UniProt
Immunogen
An immune phage display library derived from the peripheral blood lymphocytes of a llama that had been immunized with an equine vaccine that includes killed WEEV (West Nile Innovator + VEWT) was generated. The antibody was selected by panning the library against recombinant envelope (E2/E3E2) protein from WEEV.
Application Notes
The binding affinity of the VHH fragment for the WEEV E3E2 recombinant protein was measured by surface plasmon resonance (Kd= 7.2 ×10^−9 M). The ability of the antibody to detect the recombinant protein in a MagPlex sandwich immunoassay was confirmed (Liu et al., 2018; PMID: 31544894).
Antibody First Published
Liu et al. Selection of Single-Domain Antibodies towards Western Equine Encephalitis Virus Antibodies (Basel). 2018 Dec; 7(4): 44. PMID:31544894
Note on publication
The original paper describes the selection and characterization of single-domain antibodies towards the E2/E3E2 envelope protein of the Western equine encephalitis virus (WEEV).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
