Cat. No.
MABL-1463
Application
SDS-PAGE, WB, Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
EctoD2
From
Recombinant Antibody
Specificity
This antibody recognises the conserved extracellular, receptor-binding region of murine, avian and human ectodysplasin-A.
Alternative Names
O54693; Q92838; Ectodysplasin-A; EDA
UniProt
Immunogen
This antibody was raised by immunising Eda-deficient Tabby mice with an active recombinant form of EDA1 (Fc-EDA1).
Application Notes
The specificity of this antibody for EDA has been confirmed in SDS-PAGE, Western blot, ELISA, native gel electrophoresis and FC experiments (Kowalczyk-Quintas et al, 2014). This antibody blocks mammalian and avian EDA1 and EDA2 from binding EDAR (Kowalczyk-Quintas et al, 2014). When administered to pregnant wild-type mice, this antibody induces foetal ectodermal dysplasia (Kowalczyk- Quintas et al, 2014). Therapeutically, this antibody may be useful in the treatment of disorders arising as a result of excessive EDA1 action.
Antibody First Published
Kowalczyk-Quintas et al. Generation and characterization of function- blocking anti-ectodysplasin A (EDA) monoclonal antibodies that induce ectodermal dysplasia. J Biol Chem. 2014 Feb 14;289(7):4273-85. PMID:24391090
Note on publication
Describes the original generation of this antibody and its characterisation using SDS- PAGE, WB, ELISA gel electrophoresis and FC experiments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
