Cat. No.
MABL-1474
Application
in vivo, inhibition, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
ML66
From
Recombinant Antibody
Specificity
The antibody is specific for EGFR.
Alternative Names
ERBB; ERBB1; HER1; PIG61; mENA; avian erythroblastic leukemia viral (v- erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; proto- oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; epidermal growth factor receptor
UniProt
P00533
Immunogen
The original antibody was generated by immunizing female rats with membrane prep of MDA- MB468 breast cancer cell line.
Application Notes
The humanized version of the antibody exhibited strong specific binding to both human tumor cell lines and Vero cells by flow cytometry. This original and humanized version of the antibody were used for detection of EGFR expressed on MDA-MB468 cells by flow cytometry. The humanized version of the antibody induced NK cell mediated killing of target cells in a dose dependent manner. The chML66-antibody-maytansinoid conjugates significantly deleted the tumor growth in a tumor xenograft model using EGFR expressing KB cells implanted subcutaneously in SCID mice (US20160017053A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
