Cat. No.
MABL-1479
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Eimeria acervulina
Clone No.
8C3
From
Recombinant Antibody
Specificity
This antibody is specific for an unidentified surface antigen localized in the apical complex of Eimeria acervulina, a protozoan causing coccidiosis in poultry.
Alternative Names
8C3HLEK; Eimeria acervulina sporozoite
UniProt
Q24769
Immunogen
The original antibody was generated by immunizing chickens with coccidial antigens prepared from Eimeria acervulina sporozoites.
Application Notes
The original version of this antibody (chicken IgG, λ) antibody was generated and screened against surface antigens of Eimeria acervulina using ELISA (Song et al., 2001) (Kim et al., 2001; US6790937B2). A recombinant scFv version of this antibody, expressable in E. coli, was generated, screened via ELISA and characterized (Park et al., 2005; PMID: 15834788).
Antibody First Published
Song et al. Molecular Cloning and Characterization of CDNA Encoding Immunoglobulin Heavy and Light chain Variable Regions from Four Chicken Monoclonal Antibodies Specific to Surface Antigens of Intestinal Parasite, Eimeria acervulina J. Microbiol. 2001 Mar 1. 39(1):49-55 PMID:15834788
Note on publication
The original publication describes the molecular cloning and characterization of cDNA encoding immunoglobulin heavy and light chain variable regions from four chicken monoclonal antibodies developed to combat avian coccidiosis, caused by the intestinal parasite Eimeria acervulina, employing antibody engineering technology to overcome limitations associated with the hybridoma method.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
