Cat. No.
MABL-1490
Application
FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
UG1
From
Recombinant Antibody
Specificity
UG1 binds predominantly to a subset of CD14+ monocytes that mostly co-express lymphocyte markers but UG1 does not preferentially bind to cells which express receptors for proangiogenic factors.
Alternative Names
gUG1; colony-forming unit Hill cells; CFU-Hills; circulating angiogenic cells; CACs; endothelial colony-forming cells; ECFCs; early outgrowth cells; early EPCs; late outgrowth cells; late EPCs
UniProt
Immunogen
Peripheral blood was obtained from human donors. The PBMCs were obtained by density centrifugation and cultured. Two ISA brown chickens were then injected intradermally and after 50 days the spleen and bone marrow was harvested. RT-PCR was performed on cells from the spleen and bone marrow which was used to generate a cDNA library. Primers were then used to amplify the VL and VH genes and this was assembled in an scFv format. Phage libraries were generated and the library was biopanned. The biopanned library was then transformed into XL1 blue bacteria and colonies chosed at random from these.
Application Notes
Has been used in flow cytometry to show the population of cells that UG1 binds to. This was shown to be almost exclusively CD14+ and CD34- and shows no preference for cells expressing Tie-2 and CD309 which recognise angiogenic peptides. There was also a higher proportion of CD56, CD3, and CD19 (Bowes et al).
Antibody First Published
Bowes et al Developing cell-specific antibodies to endothelial progenitor cells using avian immune phage display technology. J Biomol Screen. 2011 Aug;16(7):744-54 PMID:21593485
Note on publication
Describes the generation of a library of phage displays and scFvs against a heterogeneous population of peripheral blood mononuclear cells (PBMC). It goes on to discuss the characterisation of three of these antibodies and the codon optimisation of UG1.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
