Cat. No.
MABL-1489
Application
IP, SFA, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
FB5
From
Recombinant Antibody
Specificity
Endosialin is not expressed in normal human tissues. FB5 is reactive to cultured fibroblasts and neuroblastoma cell lines, and tumour vascular endothelial cells.
Alternative Names
CD248; FB5; tumor endothelial marker 1
UniProt
Q9HCU0
Immunogen
BALB/cxA F1 mice were immunised with human fibroblasts with subsequent production and screening of hybridoma cells.
Application Notes
FB5 was used in immunohistochemistry experiments to determine the distribution of endosialin in cultured human cells and normal adult tissues (Rettig et al, 1992). FB5 was used in immunoprecipitation and Western blotting to characterise endosialin (Rettig et al, 1992). In hemadsorption rosetting assays with LA1-5s cells the concentration of FB5 required for 50% rosetting was determined as 3 ng/ml.
Antibody First Published
Rettig et al, 1992. Identification of endosialin, a cell surface glycoprotein of vascular endothelial cells in human cancer. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10832-6. PMID:1438285
Note on publication
Describes the original production of the antibody and its application to immunohistochemistry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
