Cat. No.
MABL-1495
Application
crystallisation, ELISA
Isotype
Engineer antibody
Species Reactivity
DENV
Clone No.
2D73
From
Recombinant Antibody
Specificity
2D73 reacts with residues 309-319 (DKEMAETQHGT) within domain III of the DENV envelope protein, shown crystallographically (Li et al, unpublished). Although raised against DENV-3 EDIII, 2D72 cross- reacts with EDIII from all four DENV serotypes. 2D73 does not react with other DENV-related flaviviruses, such as WNV, YFV and JEV.
Alternative Names
DENV E DIII; Dengue virus envelope protein domain III; DENV envelope protein domain III; 2D-73
UniProt
Immunogen
BALB/c mice were immunised with recombinantly-produced envelope protein domain III (EDIII) from DENV-3. Splenocytes were obtained from immunised mice and fused with the NS-1 myeloma cell line to generate hybridomas.
Application Notes
2D73 was able to strongly neutralise DENV infection in a serotype-independent manner, shown using both ELISAs and ELISPOT-based assays (Li et al, 2013). 2D73 has also been used, as a Fab fragment, to crystallise the DENV-4 EDIII protein (Li et al, unpublished).
Antibody First Published
Li et al. Dengue virus envelope domain III immunization elicits predominantly cross-reactive, poorly neutralizing antibodies localized to the AB loop: implications for dengue vaccine design. J Gen Virol. 2013 Oct;94(Pt 10):23851440
Note on publication
Describes the generation and characterisation of 25 cross-reactive anti-DENV mAbs.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
