Cat. No.
MABL-1496
Application
IP, NTRL, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
DENV-1
Clone No.
E111
From
Recombinant Antibody
Specificity
DENV1-E111 was shown to bind the E protein DIII using yeast which specifically expressed each E protein domain (Shrestha et al, 2010). It was further shown that DENV1-E111 was specific for the type 1 DENV serotype, as well as genotype 2 of this serotype. Forward genetic screens could not identify DENV-1 DIII mutations which abrogated DENV1-E111 binding, however a crystal structure of a DENV1-E111 scFv comprising the VH and VL domains showed that the mAb bound the CC' loop of DIII (Austin et al, 2012). In the same study, the authors used SPR to elucidate an affinity of 18 nM for the DENV1-E111-DIII interaction.
Alternative Names
DENV1 DIII; Dengue virus 1 envelope protein DIII domain; DENV-1 envelope protein DIII domain; DENV-1 E protein DIII domain; DENV-1 E protein DIII
UniProt
Immunogen
Immunodeficient C57BL/6 background mice were infected with DENV-1 (genotype 2). In some cases, mice were boosted by injection of recombinantly produced DENV-1 E protein DIII. Splenocytes obtained from immunised mice were then fused with P3X63Ag8.53 myeloma cells to generate a hybridoma.
Application Notes
DENV1-E111 has been shown to bind strongly to DENV-1 E protein DIII by immunostaining and flow cytometry (Shrestha et al, 2010). The mAb is a strong serotype- and genotype- specific binding agent, and so can be applied as a reliable diagnostic tool.
Antibody First Published
Shrestha et al. The development of therapeutic antibodies that neutralize homologous and heterologous genotypes of dengue virus type 1. PLoS Pathog. 2010 Apr 1;6(4):e1000823 PMID:20369024
Note on publication
Describes the generation of anti-DENV-1 mAbs by infecting mice with DENV-1 and boosting some with recombinant E protein DIII. mAb DENV1-E111 showed strong neutralisation activity specifically for the DENV type 1 serotype.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
