Cat. No.
MABL-1497
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4B5E6D7
From
Recombinant Antibody
Specificity
The antibody is specific for EP-CAM. EP-CAM is a transmembrane glycoprotein mediating Ca²⁺- independent homotypic cell–cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. It plays a role in embryonic stem cells proliferation and differentiation and it up-regulates the expression of FABP5, MYC and cyclins A and E.
Alternative Names
CGMCC No.17406; Epithelial cell adhesion molecule; Adenocarcinoma- associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor- associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD326; EPCAM; MOC-31
UniProt
P16422
Immunogen
The original antibody was generated by immunizing BALB/c mice with EP-CAM.
Application Notes
The antibody 4B5E6D7 and the commercial antibody MOC-31 were used to stain lung squamous cell carcinoma, lung adenocarcinoma, cervical cancer, colorectal carcinoma, duodenum and rectal tissues. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 4B5E6D7 was higher than the commercial antibody. The antibody and the commercially available antibody MOC-31 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN110922485B).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
