Cat. No.
MABL-1505
Application
therapeutic, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MT201 (Adecatumumab; HD69)
From
Recombinant Antibody
Specificity
This antibody is specific for human EpCAM.
Alternative Names
CD326; Ep-CAM; KS1/4 antigen; KSA; Epithelial cell adhesion molecule; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; 17-1A-antigen; EGP314; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; EGP-2; EGP40; KSA; CO17-1A; GA733-2
UniProt
P16422
Immunogen
The original antibody was generated by panning against a naturally expressed human library.
Application Notes
Immunohistochemistry was performed on human metastatic breast cancer tissue using this antibody (Schmidt et al, 2010; pmid:19633042). ELISA was performed on recombinant EpCAM protein using this antibody (Raum et al, 2000; pmid:11419181). Flow cytometry using this antibody was done on cells (Raum et al, 2000; pmid:11419181). Western blot was performed on 119 polypeptides of 13 amino acids shifted by two amino acids covering the entire extracellular domain of EpCAM. This antibody was used (Raum et al, 2000; pmid:11419181). Surface plasmon resonance was performed on the EpCAM protein using this antibody (Raum et al, 2000; pmid:11419181). This antibody was used for flow cytometery on KATO III cells (Naundorf et al, 2002; pmid:12115595). A cytotoxicity experiment was performed on PMBC using this antibody (Naundorf et al, 2002; pmid:12115595). Phase II trials were performed on prostate cancer patients (Marschner et al, 2010; pmid:20606402). Phase I trials were performed on prostate cancer patients (Oberneder et al, 2006; pmid:16930989).
Antibody First Published
Raum et al. Anti-self antibodies selected from a human IgD heavy chain repertoire: a novel approach to generate therapeutic human antibodies against tumor-associated differentiation antigens. Cancer Immunol Immunother. 2001 May;50(3):141-50. PMID:11419181
Note on publication
Human antibodies were isolated by phage display from a naturally expressed human antibody repertoire.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
