Cat. No.
MABL-1504
Application
IHC;, SPR, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MOC31
From
Recombinant Antibody
Specificity
This antibody is specific for human EpCAM.
Alternative Names
CD326; Ep-CAM; KS1/4 antigen; KSA; Epithelial cell adhesion molecule; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; 17-1A-antigen; EGP314; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; EGP-2; EGP40; KSA; CO17-1A; GA733-2; MOC-31
UniProt
P16422
Immunogen
The original antibody was generated by immunizing mice with human EGP-2 antigen.
Application Notes
The binding affinity of this antibody for human EpCAM was determined using surface plasmon resonance and ELISA. The scFv version of this antibody was used to immunohistochemically stain human carcinoma cells, colonic epithelium, colon carcinoma and melanoma (PMID: 9836471). This antibody binds EpCAM-positive Kato III cell line in a flow cytometric assay. The IgG2a format of this antibody was used for the immunohistochemical analysis of normal human tissues and cancerous tissues. In immunohistochemical studies, MOC31 showed binding towards colon cancers, breast cancer and lung cancer with epithelial origin (PMID: 24525727). This antibody is also reported to be used in differentiation between adenocarcinoma and reactive mesothelial cells (PMID: 10603193). This antibody was also used for immunohistochemical staining of adenocarcinomas (PMID: 16329115).
Antibody First Published
Roovers et al. High-affinity recombinant phage antibodies to the pan- carcinoma marker epithelial glycoprotein-2 for tumour targeting. Br J Cancer. 1998 Dec;78(11):1407-16. PMID:9836471
Note on publication
Describes the generation of high affinity recombinant phage antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
