Cat. No.
MABL-1513
Application
Blocking, inhibition, SPR, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B11
From
Recombinant Antibody
Specificity
This antibody is specific for human Ephrin-B2.
Alternative Names
EFNB2; EPLG5; HTKL; LERK5; HTK-L; LERK-5; HTK ligand; EPH-related receptor tyrosine kinase ligand 5
UniProt
P52799
Immunogen
The original antibody was raised by panning a human antibody against ephrin B2.
Application Notes
The original antibody was used for an ELISA on ephrin B2. This antibody was used for flow cytometry on specific antisera of each member of the ephrinB family (Abéngozar et al., 2012; PMID:22446484), and on human CD138+ cells (Sasine et al., 2024; PMID:38231476). This antibody was used for a western blot on different proteins from ephrinB2 family, it showed it was only able to bind to ephrinB2. This antibody was used for surface plasmon resonance to determine its binding strength. This antibody was used for a transwell migration assay and a wound healing assay, it showed that the percentage of HUVEC migrated cells was reduced from 80% (untreated) to 50% and 40%, respectively. This antibody was used for a matrigel assay on HUVEC cells, here it inhibited the formation of tube-like structures by >40%. This antibody was used in vivo on nude or SCID mice with xenografted tumors (BxPC3, SW620, H460), it showed strong tumor inhibition for all these tumor types (Abéngozar et al., 2012; PMID:22446484). This antibody was used for blocking on BM-MSC cultures (Alfaro and Zapata., 2018; PMID:29941036). This antibody was used for a migration and methylcellulose assay in vitro, here it showed it was able to block Ephrin-B2 signalling (Krusche et al., 2016; PMID:27350048).
Antibody First Published
Abéngozar et al. Blocking ephrinB2 with highly specific antibodies inhibits angiogenesis, lymphangiogenesis, and tumor growth Blood. 2012 May 10;119(19):4565-76. PMID:22446484
Note on publication
In the original paper to investigate the functional consequences of blocking ephrinB2 activity, 2 highly specific human single-chain Fv (scFv) Ab fragments against ephrinB2 were generated and characterized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
