Cat. No.
MABL-1522
Application
RIA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
15H11
From
Recombinant Antibody
Specificity
This antibody is specific for estradiol. Estradiol is an estrogen steroid hormone and the major female sex hormone. It is involved in the regulation of the estrous and menstrual female reproductive cycles.
Alternative Names
7-(O-carboxymethyl)oximinoestradiol; 17β-estradiol; oestradiol
UniProt
Immunogen
This antibody was raised by immunizing male Balb/c mice with 7-(O- carboxymethyl)oximinoestradiol-BSA.
Application Notes
A radioimmunoassay was preformed with the mouse version of this antibody on estradiol tracers (Rousselot et al, 1997; pmid:9201930).
Antibody First Published
Rousselot et al. Specific photoaffinity labeling of Tyr-49 on the light chain in the steroid-combining site of a mouse monoclonal anti-estradiol antibody using two epimeric 6alpha- and 6beta-(5-azido-2-nitrobenzoyl)amidoestradiol photoreagents. Biochemistry. 1997 Jun 24;36(25):7860-8. PMID:9201930
Note on publication
A mouse monoclonal anti-7-(O-carboxymethyl)oximinoestradiol antibody was photoaffinity labeled with two cross-reactive 6alpha- and 6beta-(5-azido-2-nitrobenzoyl)amido[17alpha- 3H]estradiol photoreagents (6alpha- and 6beta-ANBA-[17alpha-3H]estradiol).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
