Cat. No.
MABL-1523
Application
functional assay, RIA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
9D3
From
Recombinant Antibody
Specificity
This antibody is specific for the 17b-hydroxy group of estradiol. It is reported to show stronger recognition of the D-ring than of the A-ring extremity of the steroid, but a similar lack of specificity for both 6- and 7-positions of the B-ring. Estradiol is an estrogen steroid hormone and the major female sex hormone. It is involved in the regulation of the estrous and menstrual female reproductive cycles.
Alternative Names
7-(O-carboxymethyl)oximinoestradiol; 17β-estradiol; oestradiol
UniProt
Immunogen
This antibody was raised by immunizing male Balb/c mice with 7-(O- carboxymethyl)oximinoestradiol-BSA.
Application Notes
A radioimmunoassay was performed with the mouse version of this antibody on estradiol tracers (Rolland de Ravel et al, 2001; pmid:11732911). The binding specificity of the mouse version of the antibody was determined using photoaffinity labelling on different forms of estradiol (Rolland de Ravel et al, 2001; pmid:11732911).
Antibody First Published
Rolland de Ravel et al. Specific Photoaffinity-Labeling of Tyr-50 on the Heavy Chain and of Tyr-32 on the Light Chain in the Steroid Combining Site of a Mouse Monoclonal Anti-Estradiol Antibody Using C3-, C6-, and C7-Linked 5-Azido-2-nitrobenzoylamidoestradiol Photoreagents. Biochemistry. 2001 Dec 11;40(49):14907-20. PMID:11732911
Note on publication
A mouse monoclonal anti-7-(O-carboxymethyl) oximinoestradiol antibody 9D3, raised against the same immunogen as that employed for generating the reported anti-estradiol antibody 15H11 [Rousselot, P., et al. (1997) Biochemistry 36, 7860-7868], was found to exhibit an opposite specificity profile with a much stronger recognition of the D-ring than of the A-ring extremity of the steroid, but a similar lack of specificity for both 6- and 7-positions of the B-ring.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
