Cat. No.
MABL-1524
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
D12
From
Recombinant Antibody
Specificity
This antibody is specific for estradiol. Estradiol is an estrogen steroid hormone and the major female sex hormone. It is involved in the regulation of the estrous and menstrual female reproductive cycles.
Alternative Names
17-beta-estradiol; E2; ethynyl estradiol; estradiol; oestradiol; ChEBI: 16469; MSP1-D12
UniProt
Immunogen
This antibody was raised by panning against large single chain Fv library derived from lymphoid tissues including tonsil, bone marrow and peripheral blood lymphocytes.
Application Notes
The scFv version of this antibody binds estradiol with an affinity of 3.7nM in and ELISA. To assess its physical characteristics the binding affinity of this antibody was also measured using SPR on estradiol using the scFv version of this antibody (Vaughan et al, 1996; pmid:9630891). To test the binding of this antibody, an ELISA was performed on either thyroglobulinoestradiol, BSA-oestriol or BSA using the scFv version of this antibody. Further the rate of association and dissociation was determined using surface plasmon resonance. This was performed with estradiol as antigen and the scFv version of this antibody (Pope et al, 1996; pmid:9373313).
Antibody First Published
Vaughan et al. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat Biotechnol. 1996 Mar;14(3):309-14. PMID:9630891
Note on publication
Functional V-gene segments from 43 non-immunized human donors were used to construct a repertoire of 1.4 x 10(10) single-chain Fv (scFv) fragments displayed on the surface of phage. Fragments were cloned in a phagemid vector, enabling both phage displayed and soluble scFv to be produced without subcloning.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
