Cat. No.
MABL-1530
Application
EIA, functional assays, IP, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
D547
From
Recombinant Antibody
Specificity
This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c- Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling.
Alternative Names
Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1
UniProt
P03372
Immunogen
This antibody was raised by immunizing a Lewis rat, with purified estradiol-receptor complex of calf uterine nuclei
Application Notes
crude nuclear E*R of calf uterus (0.1 pmol), normal rat serum (10 Ml), and hybridoma medium were used in a double antibody immunoprecipitation with this antibody (Greene et al, 1980; pmid:6928610). ELISA was preformed with this antibody on human MCF-7 cell cytosol (Greene et al, 1982; pmid:6979662). Fresh breast cancer tumor was immunostained using this antibody (Miller et al, 1993; pmid:8490902). An EIA was preformed on cytosol from breast cancer tumors using this antibody (Cren et al, 1991; pmid:1911441). COS-7 cells cotransfected with expression plasmids encoding ERa and HA-tagged ERb were immunoprecipitated with this antibody (Ogawa et al, 1998; pmid:9473491). Sucrose Density Gradient Analysis was preformed on GH3 cells (Tate et al, 1984; pmid:6708522). Immunocytochemistry was preformed on the reproductive tract of Female cynomolgus macaques using this antibody (McClellan et al, 1984; pmid:6373239). [3H]E2-labeled macaque E2 receptors were examined on sucrose density gradients using this antibody (McClellan et al, 1984; pmid:6373239).
Antibody First Published
Greene et al. Monoclonal antibodies to estrophilin: Probes for the study of estrogen receptors Proc Natl Acad Sci U S A. 1980 Jan;77(1):157-61. PMID:6928610
Note on publication
The paper describes the antibody being raised by immunizing lewis rats with purified estradiol-receptor complex of calf uterine nuclei.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
