Cat. No.
MABL-1529
Application
therapeutic, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
S72 (scFv7)
From
Recombinant Antibody
Specificity
This antibody is specific for the amino acid residues 284-310 of human estrogen receptor (hER- α36). The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling.
Alternative Names
ER; ERα36; hER-α36; ESR1; ER-alpha; NR3A1; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
UniProt
P03372
Immunogen
This antibody was generated from a phage display library constructed from normal human lymph cells using scFv expression vectors. The scFv antibody was isolated by panning the library against a target peptide comprising amino acid residues 284-310 of human estrogen receptor (hER-α36).
Application Notes
To characterize the binding of this antibody an ELISA was performed against biotinylated ERα36 using the scFv version. The scFv version of this antibody can also recognize human ERα36 in a western blot assay. And to identify the therapeutic advantages of this antibody, BCAP-37 tumors were implanted in balb/c nude mice after which the scFv version of this antibody was administered. The tumor sizes showed a significant difference with the control group (US20110311517).
Antibody First Published
Antibodies and methods for treating estrogen receptor-associated diseases. US20110311517
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
