Anti-Estrogen receptor mAbs [JS34/32](MABL-1528)

MABL-1528

functional assay, IP, IHC

Engineer antibody

Bovine, Rat, Human

JS34/32

Recombinant Antibody

This antibody is specific for human, bovine and rat estrogen receptor. The different molecular forms of the estrogen receptor also showed no difference in binding affinity. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1

P49884

This antibody was raised by immunizing Balb/c mice with bovine estrogen receptor purified from calf uterus.

Aliquots of 16a- [ 125I]iodo17P-estradiol-labeled receptor were immunoprecipitated with the mouse IgG2b version of this antibody (Moncharmont et al, 1982; pmid:7159574). To assess the localization of estrogen receptor (ER) in rat brain, pituitary and uterus, immunohistochemistry was performed on the brain, pituitary and uterus of rats (Sar and Parikh, 1986; pmid:2422450). To test whether the role of estrogen in breast cancer is dependent on a modified expression of their receptor, immunohistochemistry was performed on samples of human breast cancer using the mouse version of this antibody (Fabris et al, 1987; pmid:3695478). Determination of antibody interaction with microsomal and cytosolic estrogen-binding proteins was carried out by monitoring the shift in sedimentation coefficient of estrogen-estrophile complexes that occurs when they are bound by the mouse version of this antibody. This was done to characterize estrogen-binding sites associated with the endoplasmic reticulum of rat uterus (Evans Jr and Muldoon, 1991; pmid:2020979). To demonstrate the existence of receptors for estradiol in rabbit articular cartilage and to measure their concentration and dissociation constants, immunoprecipitation on cell cultures from rabbit cartilage tissue was performed using the mouse version of this antibody (Dayani et al, 1988; pmid:3419165). Immunohistochemistry was performed on human breast cancer tissue using the mouse version of this antibody, to show that immunohistochemistry is a viable method to assay cancer tissue (Marchetti et al, 1987; pmid:2436766).

Moncharmont et al. Monoclonal antibodies against estrogen receptor: interaction with different molecular forms and functions of the receptor. Biochemistry. 1982 Dec 21;21(26):6916-21. PMID:7159574

Hybridoma cells have been produced by fusing SP2/O-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a purified preparation of estrogen receptor from calf uterus.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.