Cat. No.
MABL-1550
Application
functional assay, inhibit
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
EL-16
From
Recombinant Antibody
Specificity
This antibody binds the C2 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Alternative Names
AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component; IT2
UniProt
P00451
Immunogen
The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain.
Application Notes
The inhibitory activity of this antibody against factor VIII was determined using a one- stage clotting assay. This antibody was capable of completely eliminating FVIII inhibition by CLB-CAg 117, a C2 domain directed anti-factor VIII antibody (PMID: 10627462).
Antibody First Published
van den Brink et al. Human antibodies with specificity for the C2 domain of factor VIII are derived from VH1 germline genes Blood. 2000 Jan 15;95(2):558-63. PMID:10627462
Note on publication
Describes the generation and epitope mapping of antibodies against C2 domain of factor VIII.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
