Cat. No.
MABL-1562
Application
in vitro, in vivo, IP, WB, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
73.3
From
Recombinant Antibody
Specificity
The antibody is specific for fibroblast activation protein (FAP). FAP is a membrane protease that is highly expressed by cancer-associated fibroblasts (CAFs).
Alternative Names
Fibroblast activation protein; Fibroblast activation protein-α; Prolyl endopeptidase FAP; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha; FAPalpha1; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme;Serine integral membrane protease; SIMP; Surface-expressed protease; Seprase
UniProt
P97321
Immunogen
The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells.
Application Notes
A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3ζ and 4–1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35–50% following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014; PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019; PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016; PMID: 26663085).
Antibody First Published
Wang et al. Targeting fibroblast activation protein in tumor stroma with chimeric antigen receptor T cells can inhibit tumor growth and augment host immunity without severe toxicity Cancer Immunol Res. 2014 Feb;2(2):154-66. PMID:24778279
Note on publication
The paper describes the generation of the antibody and the generation of the anti-muFAP-CAR construct.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
