Cat. No.
MABL-1564
Application
crystallization, in vitro, in vivo, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
HFE7A
From
Recombinant Antibody
Specificity
This antibody is specific for human and mouse FAS.
Alternative Names
CD95; APT1; FAS1; TNFRSF6; Apo-1 antigen; FASLG receptor; Apoptosis- mediating surface antigen FAS; Tumor necrosis factor receptor superfamily member 6
UniProt
P25445; P25446
Immunogen
The original antibody was raised by immunizing a Fas-deficient mice with partially purified human Fas antigen.
Application Notes
The original antibody was used for flow cytometry on activated T cells from humans, chimpanzees, Japanese monkeys, crab-eating monkeys and marmosets, and on thymocytes from BALB/c mice. Here it was determined that this antibody was reactive to humans and mice. This antibody was used for a cytotoxic assay on synovial cells (Ichikawa et al., 2000; PMID:10744657). The structure of the original antibody was determined using x-ray crystallography (Ito et al., 2002; PMID:11754745, Haruyama et al., 2002; PMID:12499636). This antibody was used for a competitive ELISA on Fas (Haruyama et al., 2002; PMID:12499636, Yoshida et al., 2010; PMID:20024619). This antibody was used for surface plasmon resonance on mouse Fas, to determine binding affinity. The protective effects of this antibody against acute and lethal hepatic injury induced by Jo2 was assessed. It showed it was able to protect against hepatic injury. To assess the effect of this antibody on caspases in vivo, mice were injected with the antibody after Jo2-induced apoptosis of hepatocytes. It showed that it strongly inhibited activation of caspases (Yoshida et al., 2010; PMID:20024619).
Antibody First Published
Ichikawa et al. A novel murine anti-human Fas mAb which mitigates lymphadenopathy without hepatotoxicity Int Immunol. 2000 Apr;12(4):555-62. PMID:10744657
Note on publication
In the original paper they report on the interesting characteristics of the newly obtained anti-Fas mAb, HFE7A, which cross-reacts with the Fas molecules of various species ranging from human to mouse and mitigates autoimmune symptoms without hepatotoxicity in mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
