Cat. No.
MABL-1568
Application
Depletion, IP, SPR, Block, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Baboon, Capuchin Monkey, chimpanzee, Cotton-topped Tamarin, Cynomolgus, Marmoset, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey, Human
Clone No.
3G8
From
Recombinant Antibody
Specificity
3G8 binds to CD16 at an epitope which is not the IgG-binding site. This antibody cannot distinguish between CD16a transmembrane isoform found on the surface of NK cells and macrophages (which can trigger the cytolytic response), and the GPI-linked CD16b isoform present on neutrophils or soluble CD16 found in the plasma. CD16 is the low affinity IgG receptor III and binds to aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis and ADCC (antibody-dependent cell-mediated cytotoxicity).
Alternative Names
CD16; low affinity IgG receptor III; FcγRIII; low affinity FcγRIII
UniProt
Q9ULV2
Immunogen
3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.
Application Notes
3G8 antibody was used to construct a recombinant bispecific scFv (bsscFv) along with the mAb clone F3.3 anti-HLA class II antibody (connected by a (Gly4Ser)4 linker)which was expressed in SF21 insect cells. This bsscFv allows NK cells to target malignant B lymphoid cells and mediates effector cell lysis (ASCC) of the malignant cells (also lysis of primary leukaemia samples). 3G8 is also able to block neutrophil phagocytosis and stimulates NK cell proliferation, and can be used in FC, SPR, IP and IHC studies.
Antibody First Published
Bruenke et al. A recombinant bispecific single-chain Fv antibody against HLA class II and FcgammaRIII (CD16) triggers effective lysis of lymphoma cells. Br J Haematol. 2004 Apr;125(2):167-79. PMID:15059139
Note on publication
Describes the construction and characterisation of recombinant bsscFv capable of targetting CD16+ immune cells to maligant B cells resulting in ADCC.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
