Cat. No.
MABL-1573
Application
immunotherapy, IP, tumor imaging
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AMB8LK
From
Recombinant Antibody
Specificity
This antibody binds human ferritin. This protein catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form. Ferritin is important for iron homeostasis.
Alternative Names
FTMT; FTL; FTH1; Ferritin mitochondrial; B8
UniProt
Q8N4E7
Immunogen
The original antibody was generated by immunizing female Balb/c mice with ferritin extracted from human spleen cells.
Application Notes
This antibody was capable of immunoprecipitating ferritin (PMID: 6817879). The original antibody binds human ferritin with a binding affinity of Kd= 5.1nM (EP2193147). The thiolated version of this antibody was conjugated to maleimide-activated polylactide nanoparticles (NPs) to generate immunonanoparticles, which may carry drugs to desired overexpressing antigen cells potentially leading to improved drug therapy and reduced systemic adverse effects (PMID: 19835957). Preclinical studies showed that the immunoconjugate with 90Y has 98% stability over 7 days. A very good pancreatic adenocarcinoma tumor targeting was observed especially with the Bz-DTPA-AMB8LK (PMID: 17383579)
Antibody First Published
Kadouche et al. Analysis of various isoferritins with monoclonal antibodies. C R Seances Acad Sci III. 1982 Oct 18;295(6):443-8. PMID:6817879
Note on publication
Describes the generation and characterization of 4 antibodies against isoferritins.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
