Cat. No.
MABL-1574
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
F11 (HSF102)
From
Recombinant Antibody
Specificity
This antibody binds human ferritin. This protein catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form. Ferritin is important for iron homeostasis.
Alternative Names
FTMT; FTL; FTH1; Ferritin mitochondrial; B8
UniProt
Q8N4E7
Immunogen
The original antibody was generated by immunizing female Balb/c mice with ferritin extracted from human spleen cells.
Application Notes
The binding characterization of this antibody towards human spleen ferritin was done using ELISA. This antibody was also reported to show binding affinity for protein A. Binding of this antibody to ferritin causes antigen-induced conformational changes in the antibody and reduces its affinity for protein A (PMID: 9776583).
Antibody First Published
Bespalov et al. Preparation of single-chained antibodies to human ferritin in Escherichia coli. Mol Biol (Mosk). 1993 Mar-Apr;27(2):451-60. PMID:8487773
Note on publication
Describes the generation of scFv antibodies using E. coli expression system.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
