Cat. No.
MABL-1575
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4C9
From
Recombinant Antibody
Specificity
This antibody binds human acidic isoferritin. Ferritin is a protein that catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form. Ferritin is important for iron homeostasis. Ferritins occur as many isoforms, all of which are composed of various combinations of the two primary subunits, H and L. Acidic isoferritins have high proportions of H subunit.
Alternative Names
FTH1; FTHL6; AIF; acidic isoferritin; Ferritin heavy chain; Ferritin H subunit
UniProt
P02794
Immunogen
The original antibody was generated by immunizing BALB/c mice with acidic isoferritin (AIF) from primary hepatic carcinoma (PHC) tissues.
Application Notes
The binding specificity of this antibody towards acidic isoferritin, basic isoferritin and human liver ferritin was determined using ELISA. This antibody does not cross react with basic isoferritin and human liver ferritin. The binding affinity of the original mouse IgG1 format of this antibody for acidic isoferritin was reported to be Kd= 4.15 nM. This antibody was used for the immunohistochemical staining of liver tissues of primary hepatic carcinoma (PHC)(PMID: 11604110). This antibody can be used for the identification of acidic isoferritins in a western blot (PMID: 12697395).
Antibody First Published
You et al. Evaluation of clinical significance of isoferritin by development of new monoclonal antibodies specific for acidic isoferritin. Hybridoma. 2001 Aug;20(4):243-8. PMID:11604110
Note on publication
Describes the generation and characterization of two antibodies against acidic ferritin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
