Cat. No.
MABL-1581
Application
SPR, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C8
From
Recombinant Antibody
Specificity
The antibody is specific for FGFR1.
Alternative Names
CD331; FLT-2; BFGFR; bFGF-R-1; FGFR-1; N-sam; EU927378; EC:2.7.10.1;Fibroblast growth factor receptor 1; Basic fibroblast growth factor receptor 1; Fms-like tyrosine kinase 2; Proto-oncogene c-Fgr
UniProt
P11362
Immunogen
The original antibody was isolated from a naïve library by phage display by panning against FGFR1.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected FGFR1 by western blot analysis. The antibody recognized the FGFR1-GFP construct in immunofluorescence analysis on HeLa cells. The binding affinity of the original format of the antibody to FGFR1 was measured by surface plasmon resonance (Kd= 9.3 nM). The antibody was successfully employed in a sandwich ELISA (Monega et al., 2009; PMID: 19196718).
Antibody First Published
Monegal et al. Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library Protein Eng Des Sel. 2009 Apr;22(4):273-80. doi: 10.1093/protein/gzp002. Epub 2009 Feb 4. PMID:19196718
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
