Cat. No.
MABL-1582
Application
IP, RIA, WB
Isotype
Engineer antibody
Species Reactivity
Rat, Human
Clone No.
MI 7 A3
From
Recombinant Antibody
Specificity
This antibody is specific for the common FGFRl N-terminus and N-terminus of lg Loop II. FGFR is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.
Alternative Names
Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331
UniProt
P11362
Immunogen
Balb/c mice were immunized with recombinant human FGF-R1a or with both recombinant human FGF-R1b and recombinant human FGF-R1a .
Application Notes
To characterize this antibody a western blot was preformed on sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. Furthermore immunoprecipitation was preformed on the lysed sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. And finally a radioimmunityassay was preformed with the mouse version of this antibody on I125-labeled complexes (Xu et al, 1992; pmid:1325449).
Antibody First Published
Xu et al. Expression and immunochemical analysis of rat and human fibroblast growth factor receptor (flg) isoforms J Biol Chem. 1992 Sep 5;267(25):17792-803. PMID:1325449
Note on publication
A panel of 16 isoform and domain-specific polyclonal and monoclonal antibodies was developed and characterized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
