Anti-FGRIa mAbs [M2F12](MABL-1589)

MABL-1589

IP, RIA, WB, IF, IHC

Engineer antibody

Rat, Human

M2F12

Recombinant Antibody

This antibody is specific for residues 30-74 of a-specific Ig-like loop 1 from human FGF-R. FGFR is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.

Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331

P11362

Balb/c mice were immunized with recombinant human FGF-R1a or with both recombinant human FGF-R1b and recombinant human FGF-R1a .

To characterize this antibody a western blot was preformed on sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. Furthermore immunoprecipitation was preformed on the lysed sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. And finally a radioimmunityassay was preformed with the mouse version of this antibody on I125-labeled complexes (Xu et al, 1992; pmid：1325449). During research on the localization and behaviour of the FGF-1 protein the murine version of this antibody was used to immunoprecipitate FGFR and a 40-kDa complex it is part of (Feng et al, 1995; pmid：9244177). To research the localization of FGF-1 the murine version of this antibody was used to immunoprecipitate FGFR-1 in NIH3T3 cells and L6 cells (Prudovsky et al, 1994; pmid：7527394). While researching the aberrant branch formation of granule cell axons (mossy fiber sprouting) in the dentate gyrus of many patients with temporal lobe epilepsy and in animal models of epilepsy. The murine version of this antibody was used for immunocytochemistry and immunohistochemistry on cultured granule neurons from P3 sprague dawley rats (Shimada et al, 2016; pmid：27098696). To study FGF expression in RCC tissue immunohistochemistry was preformed on prospectively collected surgical samples of the tumor tissue using the murine version of this antibody (Volkova et al, 2021; pmid：33816695). To investigate the distribution and immunolocalization of fibroblast growth factors. Oviduct tissue from wistar rats was immunohistochemically analyzed using the murine version of this antibody (Alan and Liman, 2020; pmid：33772852). FGFR1 immunoreactivity was analyzed in frozen sections of normal human brain white matter and glioblastomas by using the murine version of this antibody (Morrison et al, 1994; pmid：8168112).

Xu et al. Expression and immunochemical analysis of rat and human fibroblast growth factor receptor (flg) isoforms J Biol Chem. 1992 Sep 5;267(25)：17792-803. PMID：1325449

A panel of 16 isoform and domain-specific polyclonal and monoclonal antibodies was developed and characterized.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.