Cat. No.
MABL-1592
Application
ex vivo, in vivo, ELISA, Imaging
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
8E 5
From
Recombinant Antibody
Specificity
8E5 binds to the amino-terminal region of the β-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. The epitope recognised by this clone is only accessible upon conversion of fibrinogen to fibrin, which entails the proteolytic removal of fibrinopeptides A & B from fibrinogen by thrombin. The antibody is there is therefore fibrin specific. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.
Alternative Names
Factor Ia; factor-1a; fibrin; fibrin β chain; fibrin beta-chain; fibrin beta chain; fibrin β chain; Fibrinopeptide B
UniProt
P02675
Immunogen
8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic β-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).
Application Notes
The recombinant scFv version of 8E5 retains the same antigen-binding activity of the parent antibody. 8E5 can bind to fibrin in the monomeric and polymeric form, both in vivo and ex vivo. This antibody can also be used for the immunodetection of fibrin in vivo. Standard ELISA and competitive ELISA using the epitope peptide has been conducted with both the parental antibody and the scFv.
Antibody First Published
Song et al. Primary structure and functional expression of heavy- and light- chain variable region genes of a monoclonal antibody specific for human fibrin. Hybridoma. 1997 Jun;16(3):235-41. PMID:9219033
Note on publication
Describes the derivation of the scFv of 8E5 and the determination of the protein sequence of the variable regions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
