Cat. No.
MABL-1593
Application
Biacore, WB, ELISA, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
A2
From
Recombinant Antibody
Specificity
This antibody binds to the ED-B domain of human fibronectin.
Alternative Names
FN; EDB(+) FN; ED-B domain of fibronectin; Extra domain-B containing fibronectin
UniProt
P02751
Immunogen
2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells.
Application Notes
Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Extra domain-B containing fibronectin has been proposed marker of angiogenesis, has been shown to be expressed in a number of human cancers and in ocular neovascularization in patients with proliferative diabetic retinopathy.
Antibody First Published
Pini et al. Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel. J Biol Chem.1998 Aug 21;273(34):21769-76. PMID:9705314
Note on publication
Describes generation, using phage display, of antibodies with subnanomolar affinity against markers of angiogenesis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
