Cat. No.
MABL-1597
Application
RIA, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
L19 (Radretumab)
From
Recombinant Antibody
Specificity
This antibody is specific for human fibronectin; it binds an epitope within the ED-B domain.
Alternative Names
FN; Cold-insoluble globulin; CIG; EDB(+) FN; ED-B domain of fibronectin; Extra domain-B containing fibronectin
UniProt
P02751
Immunogen
The original antibody was generated using phage display from a human antibody library.
Application Notes
The original version of this antibody (human scFv) was determined to bind human fibronectin at the ED-B domain with a Kd of 54 pM using SPR. Furthermore, the antibody forms a stable complex with the fluorescently labeled ED-B and exhibits a long half-life when complexed with it (Pini et al., 1998; PMID: 9705314). This antibody was used for ELISA on biotinylated ED-B. Furthermore, it was used for a radioimmunoassay conducted on mice with subcutaneously implanted murine F9 teratocarcinoma, and for immunohistochemistry and Immunofluorescence performed on the corneas of New Zealand white rabbits (US8097254). Various formats of this antibody were used in an ELISA on the ED-B domain of FN (Borsi et al, 2002; pmid:12353237). This antibody was used for immunohistochemistry on tissues from patients with HNSCC (Birchler et al., 2003; PMID: 12838025). A radiolabeled version of this antibody was used for SPECT/CT on patients with SCC (Birchler et al., 2007; PMID: 17418248).
Antibody First Published
Pini et al. Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel. J Biol Chem.1998 Aug 21;273(34):21769-76. PMID:9705314
Note on publication
The original publication describes the construction and utilization of a phage display human antibody library, employing protein design principles to produce antibodies — including L19 — with subnanomolar affinity against the ED-B domain of fibronectin, a marker of angiogenesis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
